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Simultaneous Detection of Insecticides and Growth Regulators by Single Chromogenic Spray Reagents
Authors: N.L.CHUTKE, R.B.LANJEWAR, M.R.LANJEWAR
Number of views: 528
Insecticides are widely used in India for agriculture purpose1-2. Due to easy
availability of insecticides with new trade names and mixture of different class they are
knowingly or unknowingly used for homicidal and suicidal poisoning purposes. Thin
layer chromatography is found to be the best option for the identification of these
insecticides. Presently number of chromogenic reagents is used to identify different class
of insecticides from the biological materials which is found to be tedious, laborious, time
consuming and increases the analytical cost. Hence a single unique chromogenic spray
reagent for detection of different commonly used insecticides like organophosphorous,
carbamate, organochloro, sysnthetic pyrethroids and plant growtrh regulators by thin
layer chromatoraphy technique. The present paper report if 10% of aqueous sodium
hydroxide and 0.1% Bromophenol Blue in 1% silver nitrate solution in absolute alcohol
at room temperature sprayed on these insecticide gives different colour spots like blue,
purple, yellow, black green for different insecticides. Limit of detection of these reagents
is about 10μg.
Keywords: suicidal, Homicidal