The Journal of V. N. Karazin Kharkiv National University. Series «Medicine»

10-18

Authors: A.V. Martynenko, M.I. Yabluchansky

The proposed classification of dynamic HRV processes is based on M-indexes: |М|<0.03 – linear, station-ary; 0.3>|М|>0.03 – weakly nonlinear, quasi stationary; 3>|М|>0.3 – nonlinear, transient; |М|>3 – strongly nonlinear, transient. The character of M-index gives following interpretation for direction of dynamic HRV process (integrated for analyzing record): «+» – accelerating; «–» – decelerating. Thus M-indexes introduce generalized basis for allocation of HRV stationary processes: М0 indexes – determine as stationary the whole class on inertial movements in HRV, i.e. movement with constant speed or stochastic vibrations around un-changed frequency; М1 indexes – determine as stationary the whole class of periodical and quasi periodical movements in HRV, the movements which keep constant phase flow what can be achieved by introducing of time arrow; М indexes – consider the whole system of appointed above stationary processes in HRV.

24-30

Authors: A.Yu. Petrenko, G.A.Kovalyov

The action of cryopreserved cells of embryonic liver (CEL) on the model of alcohol induced hepatic in-jury at rats was explored. It is shown that intravenous injection of CEL improves the organism’s general state (enlarges the increase of body weight, improves the hair state) and restores the functional state of liver (normalizes duration of hexenal sleep and prothrombin time, increases albumin content in blood plasm). Indicated results, got on a background of continued alcohol intake.

35-42

Authors: M.I. Yabluchansky, A.V. Martynenko

The method extending spectral analysis of heart rate variability (HRV) application for patients with sinus arrhythmia was proposed. The method is based on Lyapunovs’ central theorem of spectral analysis that bands together total power (TP) and integral from quadratic of function. Рroposed method exactly split mixed model signal on true independent sources; that we obtain stochastic and harmonic sources when we split real HRV records; that TP of harmonic source good correlated with expected TP value for patients’ age group. Thus method extends HRV technology for medical practice.

47-53

Authors: S.A. Bychkov

A new method of the laparoscopic subtotal cholecystectomy it is developed. Its efficiency is confirmed on 30 experimental rabbits of the sort Chinchillas. Author offers his experience of laparoscopic subtotal cholecystectomy execution at 34 patients with acute cholecystitis. The analysis of results of experimental researches showed safety and efficiency of the laparoscopic subtotal cholecystectomy application with the cryodestruction of mucous membrane of remaining part of back wall of gall-bladder.

58-62

Authors: I.A. Bondarenko

The relation between clinical signs, parameters of the heart rate variability (HRV) and function of an external breathing (FEB) was studied in the patient with chronic obstructive pulmonary disease (COPD) before and in 1 month of therapy. 74 patients with II-IV stages of COPD were investigated. The health history, the data oh physical examination, laboratory and instrumental data were examined. The estimated parameters of FEB were next: respiration rate (RR), forced exhalation volume per 1 second (FEV1), relation of FEV1 to forced vital capacity. Among the HRV parameters, we estimated heart rate (HR), total power (ТР) and its components in domains of very low frequencies (VLF), low frequencies (LF), high frequencies (HF) and LF/HF relation. The results shows clinical symptoms of COPD improved with inauthentic increase of FEB parameters and ТР HRV increase in the presence of LF/HF decrease on therapy stage.

66-70

Authors: O.V. Grishchenko, I.V. Lakhno, A.T. Ovcharenko, V.L. Dudko

Venous vessels incompetence during pregnancy is assotiated with decreased venous drainage from intervillous space, myometrial sinuses and parametrial veins. These changes are connected with fetoplacental insufficiensy which is due to the systemic inflammafory response. The inflammation induces disorders of connective tissue and cell membranes of fetoplacental system. We have investigated the theraputical effectiveness of bilberry vita complex and venotonic drug phlebodia in pregnant women with fetoplacental insufficiensy. We have observed 64 patients with fetoplacental insufficiensy at term of pregnancy 34-36 weeks and 37 of them were taken bilberry vita complex 1 capsule twice per day and phlebodia 1 pill once daily. In all inverstigated patients were determined the activity of lipid peroxidation with biochemiluministry of blood serum method and intrauterine fetus well-being assessment. We have proved that fetoplacental insufficiensy was accompanied with oxidative stress that have promoted to the destruction of connective tissual structures in venous vessels. It was charactetized with low uteroplacental hemodynamics and chronic fetal distress. The application of bilberry vita complex and phlebodia have improved hemocirculation in the vessels of small pelvis, placenta and fetus and fetal well-being in higher degree than traditional therapy.

75-77

Authors: M.I. Lutai, А.N. Lomakovskyi, T.I. Gavrylenko, S.I. Deyak

We have established that 127 patients with stable angina pectoris (III-IV grade) and confirmed stable plaque diagnosed by angiography demonstrated a higher level of the inflammatory markers (C-reactive protein, interleukins-6, -4, -10, -8; interferons; cell inflammatory reactions) which are responsible for chronic immune inflammation. The activity of systemic inflammation is not associated with the number of affected vessels and disease duration, but more considerable manifestations of immune inflamination occurs in patients with a high functional class of angina pectoris.

84-88

Authors: N.N. Popov, E.V. Ognivenko, E.A. Romanova

The purpose of the research was the study of immune disoders in patients with acute purulent maxillitis, the study of general and local immune reactions before and after treatment. 42 patiennts with acute purulant maxillitis have been studied. They were divided into 2 groups: the 1-st group consisted of 22 patients with maxillitis without complications. The 2-nd group (20 patients) had maxillitis with complications. The acute purulent maxilitis in the 1-group is proceed with local and general immunity factors activation. Patients from 2-nd group didn’t show immune factors’ activation.

92-96

Authors: I.А. Sudoma

The problem of endometrial receptivity remains to be one of the most actual in the field of human rep-roduction. The embryo can implant during the narrow period called the «implantation window» which is cha-racterized by formation of pinopodes. The aim of this study was to evaluate the pinopode formation in healthy oocyte donors in the cycles of controlled ovarian hypestimulation (COH). The objects of investigation were 20 healthy oocyte donors. The pinopodes in the endometrial biopsies were investigated with scanning electron microscopy. In the majority of the cases (13-65%) developed pinopodes were found on LH+6 and LH+7, in 7 women (35%) – on LH +8 – LH+10. In repeated COH cycles in the same women the developed pinopodes were seen on the same days in 5 (from seven) cases and with 1-day difference in 2 cases. In COH cycles pinopodes appear on the LH+6 – LH+10 days; in the majority of cases the developed pinopodes are seen on LH+6, LH+7; the time of pinopode formation is mainly constant in the same woman under the same conditions; COH does not influence the time frames of pinopode formation.

100-105

Authors: V.S. Chepurna

The article includes the experimental research of Khatka-Yoga health-improving gymnastics influence to the dynamics activities function of respiratory system of schoolchildren. It is shown effectiveness of the Khatka-Yoga health-improving gymnastics.

117-119

Authors: Yu.M. Solovjova

In the article there is a short description of the main systems of the body-oriented psychotherapy, both known and new. Body-oriented psychotherapy becomes very close to the medicine, having the same subject of research and exerting efforts – the human body. In modern systems, for example, in Bodynamic Analysis, these two sciences practically intersect. Bodily feelings, pain, the structure of the muscular shell become a diagnostics in psychotherapy, and a work with them leads to the healing of many psychosomatic diseases.

9-18

Authors: L.S. Godlevsky, B.A. Lobasyuk, E.V. Kobolev, van E.L.J.M. Luijtelaar, A.R.M.L. Coenen, K.I. Stepanenko, Raz Haghoel, T.V. Prybalovetz

Relationships between amplitude of penicillin-induced generalized epileptiform signals in different zones of the brain cortex (occipito-frontal bilateral leads, as well as occipital and frontal bipolar leads) were investigated in Wistar rats using multiple linear regression method of analysis. Results were expressed in the form of policycle multigrafs (multidimensional presentation) with the identification of significant (p<0,1) negative or positive influences of certain direction between sources of signals. The prevalence of the involvement of left hemispheral cortex into creation of positive type of forward, and back-directed influences was identified, while greater ability to be involved in the creation of negative type of relationships was established for the occipital zone of cortex during 6-h course of generalized seizure activity development. Final stage of the epileptic activity development was characterized by positive relationship’s domination. Typical absence spike-wave activity in WAG/rij rats was characterized by the prevalence of positive relationships, which were present between almost all inspected zones of cortex. Besides, minimization of mutually negative relationships when compared with generalized penicillin-induced epilepsy, was also characteristic for WAG/rij ECoG activity. These facts might be in favor of the most pronounced synchronized processes in comparison with epileptic discharges induced by excessive dosage of penicillin solution. Authors came to conclusion that policycle presentation of relationships between amplitude signals, which are generated in different cortical zones, with an emphasis on polifunctional dependence of each investigated index might be of interest for the characteristics of experimental epilpetic process.

24-27

Authors: E.V. Sirenko

The ability of TG “Rosa”, MK and MC in the 1/100 and 1/1000 LD50 to inhibit processes of ORP, bioenergetics’ and synthesis processes was presented. It was registered the morphological metabolic lesions in the brain tissue: the hemostasis, alveolar edema, congestive edema, neuropathy and destroying levels of ARN and carbohydrates. This data is correlation whis terminal destruction of tissue.

33-39

Authors: N.I. Sklyar

The study aimed to investigate peculiarities of microbial interactions in biocenoses of stomach and duo-denum mucosal membranes at inflammatory-ulcer pathology of gastroduodenal tract. It was determined availability of antagonistic properties in 74 strains of opportunistic microflora (A. viridans, Вacillus spp, Corynebacterium spp, Pseudomonas spp, Staphylococcus spp and others). It was found that, in opposite to monocultures, complex associations of urease positive microorganisms from mucosal membranes bioptates (tissue urea determination) as well as designed microbial associations which included representatives of isolated mucosal microflora showed stimulating effect on the urea decomposition rate. Antibiotic sensitivity of microorganisms in complex microbial associations and in separate associants was shown to be significantly different: the latter showed higher sensitivity to antimicrobial preparations.

43-46

Authors: V.V. Boyko, Yu.V. Avdosjev, M.Yu. Sizy

We have treated 15 ill persons with long esophageal stricture after burn by the method of balloon dilatation. More than 70% of patients had stricture with more than 8 сm length and this was proved by X-ray method. The most of the patients had dysphagia of III-IV level. During treatment we made the dysphagia level less. During the balloon dilatations we were seen that the main reason of person’s suffering was not the whole stricture, but its main rigid part with high density and with length not more than third part of the whole stricture.

51-57

Authors: O.A. Vlasenko

It has been studied the condition of systolic and diastolic function of left ventricular in 115 patients with postinfarction cardiosclerosis at recovery stage. After 2 months of myocardial infarction it has been found the thickening of muscular concentric remodeling in 14,8%, concentric hypertrophy – 53% and excentric hypertrophy in 32,2% of patients. Dyastolic kind of chronic heart insufficiency has been determined in 47% panients, systolic – in 23,8%, dyastolic with systolic – in 28,6% patients. In all form of remodeling there was decrease of dyastolic depression and atrial filling as well. Maximum decrease of dyastolic depression was observed at patients with excentric heart hypertrophy.

62-68

Authors: G.N. Danilenko, Zh.V. Sotnikova

The purpose of the conducted research was determination of life quality subjection of rural adolescents from the complex of social hygienic factors of environment and their health’s state subjective estimation.162 pupils of ninth - eleventh forms of monosexual lyceum with the pedagogical type of education took part in researches. The Russian adapted variant of the general questionnaire SF-36 was used during researches of quality of life and conducted system analysis by the method of correlation structures. The age conditionality of indeces of physical, social functioning, bodily pain and inter-correlation of indeces characterizing quality of life was set.

73-78

Authors: S.V. Kalinichenko, O.B. Kolokolova, E.M. Babich, F.V. Kivva, O.I. Kovalenko, L.G. Verezub, L.N. Tkach, E.G. Peretiatko, L.A. Jdamarova

The sensitivity to antibiotics of causative agents of diphtheria and whooping-cough after irradiation by millimeter waves of radiofrequence range has been studied. The dependence of Corynebacterias resistance to antimicrobial preparations from phases of cultures’ growth is marked. The irradiation of Corynebacteria strains by millimeter waves resulted to increase of zones of microbial growth inhibition interdentally from the frequency of irradiation. The sensitivity to antibiotics of microorganisms of Bordetella genus after irradiation changed in different directions.

81-85

Authors: T.I. Magdaliz

It was examined endothelin-1 content in 65 patients with chronic cardiac insufficiency (CCI) caused by ischemic heart desease. In patients with CCI functional class IV was deternined the highest level of endothelin-1. Enalapril and lozartan supressed the levels of endothelin-1 in peripheral venous blood.

90-95

Authors: S.A. Pashkevich, G.N. Danilenko, A.A. Besedina

250 first form pupils were under the supervision. Health level on the basis of indeces of physical development, physical health functional reserves and peculiarities of cardio vascular system of first form schoolchildren were estimated. Analysis of indeces showed the unevenness of functional possibilities’ development of the main adaptations systems and tension of functional reserves of organism of the first form pupils in the process of teaching. Beginning of girls teaching was accompanied by tension of regulators mechanisms, and at the end of school year the satisfactory adaptive reaction was formed on the systematic loadings at a negative tendency in the indeces of physical development. At boys with more proper indeces at the beginning of teaching considerable tension of functioning of the regulatory systems is exposed at the satisfactory indeces of physical development at the end of school year.

99-105

Authors: I.А. Sudoma

Standardization of IVF cycles and ignoring of peculiarities of diseases, which leads to infertility could be the cause of low effectiveness of treatment. The aim of this work was to investigate and compare the results of IVF programs of the patients with adenomyosis and tubal-peritoneal infertility. The results of 202 IVF cycles in 136 patients with adenomyosis (main group – MG) and 92 cycles in 68 patients with tubal-peritoneal infertility (group of comparison CG) were analyzed in relation to cycle quantity,age, embryo quantity. Pregnancy rate was significantly lower in MG in comparison to CG (14,8% and 26,1% ), especially low effectiveness was seen in patients with adenomyosis and endometriosis (8,5%). Most of pregnancies were achieved after first two cycles on MG and CG (90% и 83,3%) and with young women (around 90%). The best results in MG were achieved after transfer of not more than three embryos and in CG – after transfer from three to six embryos. The analysis of IVF programs in patients with adenomyosis shows that the reproductive capacities of these women are worse if compared with patients with tubal-peritoneal infertility. In IVF programs of these patients such peculiarities must be taken into account: not more than two standard IVF programs must be fulfilled, not more than three embryos have to be transferred, after two IVF failures the more accurate diagnosis must be provided to reveal the factors which interfere with achieving pregnancy.

109-114

Authors: M.I. Yabluchansky, O.V. Ignatkina

The efficacy of the chronic heart failure (HF) therapy by enalapril maleat and metoprolol (MT) in clinical-haemodynamic indexes and parameters of the heart rate variability (HRV), depending of the initial values of total power (TP) of the HRV, at 98 patients were studied. The obtained results show that patients with HF need addition of MT as soon as possible, especially for the patients with lowest TP HRV level.