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Cytomorphometrical Analysis of Exfoliated Buccal Mucosal Cells: Effect of Smoking
Authors: Sumit Babuta1, Rohin Garg2, Khushboo Mogra3, Neha Dagal4 1Assistant Professor, Department of Anatomy, SMS Medical College, Jaipur, Rajasthan, India, 2Assistant Professor, Department of Anatomy, Teerthanker Mahaveer Medical College & Research Centre Moradabad, U.P., India, 3PG Resident, Department of Anatomy, SMS Medical College, Jaipur, Rajasthan, India, 4Senior Demonstrator, Department of Anatomy, SMS Medical College, Jaipur, Rajasthan, India
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Introduction: Exfoliative cytology is a non-aggressive, non-invasive procedure with higher patient compliance and is therefore, an
attractive technique for the early diagnosis of oral lesions. Th e purpose of this study is to evaluate and compare cytological changes using
morphometric analysis of the exfoliated buccal mucosal cells in smokers, with results obtained for non-smokers. Methods: Smears
were collected from the clinically normal buccal mucosa of 120 individuals. Age range of subjects taken was 40-60 years. Smears
were then stained with Papanicolaou stain. Results: Mean NA for smokers was signifi cantly elevated compared with the mean NA
for non-smokers. Mean CA in smokers was decreased as compared to non-smokers but the diff erence was not signifi cant. Also,
N/C ratio was signifi cantly elevated in smokers group. With increasing heavy exposure in duration of years, Cytomorphometric
changes show signifi cant altered values for all three measured parameters (NA, CA and N/C ratio). Conclusion: Increase in NA
and decreased CA as well as altered N/C ratio would appear to be due to smoking tobacco. Cytomorphometric analysis can be
used regularly to detect these cell alterations. Th is method can also aid in motivating individuals to withdraw from adverse eff ects
of tobacco smoking. Currently, use of exfoliative cytology has increased as an adjunct to screening of precancerous lesions and
malignancies of the oral cavity.
Keywords: Cytomorphology, Exfoliative cytology, Oral mucosa, Tobacco smoking