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Relation between Integrins Forced Oscillation, Degree of Actin Cytoskeleton Polymerization and Content of Free Ca2+ of Fibroblasts
Authors: Ekaterina V. Kot, Yurij G. Kot, Maria A. Grytsenko, Liubov V. Altukhova, Yevgen E. Persky
Number of views: 369
The impact of integrins oscillation on intracellular calcium in conditions of native actin microfilaments network and under depolymerization was studied on the model of α(2)β(1)-integrins forced cyclic fluctuations with the use of integrin-specific magnetic microparticles, that were exposed to magnetic field after being cell-attached. It proved that α(2)β(1)-integrins are involved in the response of fibroblasts to mechanical deformation, activating depot-dependent calcium efflux in cytoplasm. Cytoskeletal actin microfilaments are also involved in the response via these receptors, which integrity is essential for normal functioning of the depot-dependent mechanism of Ca2+ efflux in cytoplasm of deformable fibroblasts.